Toxicological Research : eISSN 2234-2753 / pISSN 1976-8257

Fig. 1.

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Fig. 1. TMEM39A expression in glioblastoma (GBM) cell lines. (A) Lysates were prepared from four established GBM cell lines (U87-MG, U251-MG, U373-MG, and U343-MG) and one established non-GBM cell lines (HEK-293A). These samples were subjected to Western blotting using anti-TMEM39A and anti-actin antibodies. The results are representative of those of three independent experiments (top panel). Relative densities were obtained by densitometry. Relative differences in TMEM39A expression levels (and the associated statistics) were calculated by normalizing all densitometric values to that of actin (in each lane) and setting the values from HEK-293A cells to 1 (bottom panel). Results are presented as the means ± SDs of data from three independent experiments. (B) Total RNA extracted from each GBM cell line was analyzed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using human TMEM39A-specific primers, as described in Materials and Methods. The results are presented as means ± SDs of data from three independent experiments. *p< 0.05, **p< 0.01.
Toxicological Research 2017;33:63~69
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