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Fig. 2.

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Fig. 2. Effect of estrogen response element (ERE) luciferase promoter activity induced by Cynanchum wilfordii Hemsley, Phlomis umbrosa Turczaninow, and Angelica gigas Nakai extract (CPAE) in MCF-7 cells. MCF-7 cells were transfected with pERE-Luc, treated with CPAE (100 μg/mL), estradiol (E2; 10 nM), ICI 182,780 (ICI, 10 μM) or a combination of these compounds for 24 hr. Cells were harvested and assayed for luciferase activity, which was normalized to β-galactosidase activity. All experiments were performed in triplicate. Bars represent mean ± SD. #P < 0.05 vs. control and *P < 0.05 vs. E2.
Toxicological Research 2017;33:71~77
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