Toxicological Research : eISSN 2234-2753 / pISSN 1976-8257

Fig. 2.

Download original image
Fig. 2. Effect of estrogen response element (ERE) luciferase promoter activity induced by Cynanchum wilfordii Hemsley, Phlomis umbrosa Turczaninow, and Angelica gigas Nakai extract (CPAE) in MCF-7 cells. MCF-7 cells were transfected with pERE-Luc, treated with CPAE (100 μg/mL), estradiol (E2; 10 nM), ICI 182,780 (ICI, 10 μM) or a combination of these compounds for 24 hr. Cells were harvested and assayed for luciferase activity, which was normalized to β-galactosidase activity. All experiments were performed in triplicate. Bars represent mean ± SD. #P < 0.05 vs. control and *P < 0.05 vs. E2.
Toxicological Research 2017;33:71~77 https://doi.org/10.5487/TR.2017.33.1.071
© ToxicolRes