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Fig. 4.

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Fig. 4. Intensities of reverse type I binding spectra of CYP1B1 with 27 flavonoids (A) and inhibition by these flavonoids of EROD activities catalyzed by CYP1B1 (B), 1A1 (C), and 1A2 (D), flurbiprofen 4-hydroxylation activities catalyzed by CYP2C9 (E), midazolam 4-hydroxylation activities catalyzed by CYP3A4 (F). The spectral changes are shown as spectral binding efficiency (ΔAmax/Km values). IC50 values are shown to be 0~1.0 μM for CYP1B1, 1A2, and 1A1, and 0~30 μM for CYP2C9 and 3A4. Abbreviations used; 3HF, 3-hydroxyflavone; 5HF, 5-hydroxyflavone; 7HF, 7-hydroxyflavone; 57DHF, 5,7-dihydroxyflavone; 357THF, 3,5,7-trihydroxyflavone; 457THF, 45,7-trihydroxytrihydroxyflavone; 457THIF, 4,5,7-trihydroxyisoflavone; 457THFva, 4,5,7-trihydroxyflavanone; 457THFvaG, 4,5,7-trihydroxyflavanone glycoside; 567THF, 5,6,7-trihydroxyflavone; 3457TetraHF, 3,4,5,7-tetrahydroxyflavone; 33457PHF, 3,3,4,5,7-pentahydroxyflavone; 4M57DHF, 4-methoxy-5,7-dihydroxyflavone; 4M57DHisoF, 4-methoxy-5,7-dihydroxyisoflavone; 2MF, 2-methoxyflavone; 3MF, 3-methoxyflavone; 4MF, 4-methoxyflavone; 34DMF, 3,4-dimethoxyflavone; 2M57DHF, 2-methoxy-5,7-dihydroxyflavone; 3M57DHF, 3-methoxy-5,7-dihydroxyflavone; 34M57DHF, 34-dimethoxy-5,7-dihydroxyflavone; 2M78DHF, 2-methoxy-7,8-dihydroxyflavone; 3M78DHF, 3-methoxy-7,8-dihydroxyflavone; 4M78DHF, 4-methoxy-7,8-dihydroxyflavone; and 34M78DHF, 3,4-dimethoxy-7,8-dihydroxyflavone. Data are taken from Shimada et al. () with modification.
Toxicological Research 2017;33:79~96
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