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Fig. 3.

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Fig. 3. Expression levels of CB1 protein in Pichia pastoris. Expressed proteins were analyzed over time at various methanol concentrations (0.5 and 1.0%). Expressed protein level was determined by (A) Coomassie blue staining and (B) western blot. (C) The protein was purified using a Ni-column (upper panels, number: fraction number, FT: flow-through, Sup: supernatant, lane 4: size marker) and gel chromatography (lower panels, Sup: supernatant, lane 2: size marker, number: fraction number). The CB1 protein size is 75 kDa.
Toxicological Research 2019;35:37~44
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