Toxicological Research : eISSN 2234-2753 / pISSN 1976-8257

Fig. 4.

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Fig. 4. Immobilization of CB1 on the biosensor chip (CM5) by amine coupling. EDC [0.4 M 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide] and NHS (0.1 M N-hydroxysuccinimide) coupling solution was used to activate the CM5 chip. CB1 protein was diluted in sodium acetate buffer (pH 5.0) and flowed into the biosensor chip at 10 μL/min for 7min. Uncoupled protein was washed out by 1M ethanolamine-HCl (pH 8.5). Proteins were coated at 2,000 response units (RU).
Toxicological Research 2019;35:37~44
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