Fig. 1. Effect of 1,2-dichloropropane (1,2-DCP) on skin thickness in C57BL/6J mice. (A) Experimental schedule for drug treatment. After 1 day of acclimation, the dorsal hair of mice was completely removed, followed by treatment with the various concentrations of 1,2-DCP (0, 2.73, 5.75, and 8.75 mL/kg) on the dorsal skin and both ears of mice, once a day for 7 days (indicated as an black arrow), while the control group was treated with corn oil without 1,2-DCP. After 7 days, the mice were sacrificed and dorsal skin was collected for subsequent experiments. (B) Image of the first day of the experiment, immediately after shaving (above), and dorsal image of mice after 7 days of daily treatment with 1,2-DCP (below). (C) Ear thickness was measured with a micrometer. Columns represent group means ± SD of ear fold thickness measurements at day-7 (D) Skin histopathological findings following treatment with 1,2-DCP. All sections were stained with hematoxylin and eosin. Mice were treated with 0, 2.73, 5.75, and 8.75 mL/kg 1,2-DCP. Epidermal thickening was observed (20×, scale bar = 10 μm). The results are means ± standard deviation (SD) of two independent experiments. *p < 0.05; **p<0.01.
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