Fig. 2. Effects of 1,2-DCP on the expression of pro-inflammatory cytokines in 1,2-DCP-treated mice. (A) Immunohistochemistry (IHC) of interleukin (IL)-6 in dorsal skin treated with 1,2-DCP (brown staining considered positive; 400×, scale bar = 10 μm). (C) IHC of skin tumor necrosis factor (TNF)-α in dorsal skin treated with 1,2-DCP (brown staining considered positive; 400×, scale bar = 10 μm). (B, D) Quantification of IHC-positive areas in the skin is shown on the right. The data were analyzed by Image-Pro Plus software (Media Cybernetics, Silver Spring, MD, USA). The results are means ± SD of two independent experiments. **p<0.01.
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