Fig. 5. 1,2-DCP induces inflammation and activates angiogenic signals in NHFD cells. (A) NHFD cells (1 × 106) were grown in 3.5 mm diameter cell culture dishes and treated with 1,2-DCP at doses of 0, 2.73, 5.75, and 8.75 μL/mL for 24 hr and imaged. (B, E) Western blot analysis of IL-6, TNF-α, VEGF, p-PKB, and p-mTOR following 1,2-DCP treatment in NHFD cells. Actin was used as a loading control. The graph shows densitometric quantification of bands. (C, D, F–H) The results are means ± SD of two independent experiments. *p < 0.05; **p < 0.01.
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