Superoxide Dismutase Activity in Small Mesenteric Arteries Is Downregulated by Angiotensin II but Not by Hypertension
Toxicol. Res. 2018;34:363−370
Published online October 15, 2018;  https://doi.org/10.5487/TR.2018.34.4.363
© 2018 Korean Society of Toxicology.

Kyu-Tae Kang1, Jennifer C. Sullivan2,3 and Jennifer S. Pollock3,4

1College of Pharmacy, Duksung Innovative Drug Center, Duksung Women’s University, Seoul, Korea
2Department of Physiology, Augusta University, Augusta, GA, USA
3Medical College of Georgia, Augusta University, Augusta, GA, USA
4Section of Cardio-Renal Physiology and Medicine, Division of Nephrology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA
Kyu-Tae Kang, College of Pharmacy, Duksung Innovative Drug Center, Duksung Women’s University, 33, Samyangro 144-gil, Dobong gu, Seoul 01369, Korea
E-mail: ktkang@duksung.ac.kr
Received: August 31, 2018; Revised: September 9, 2018; Accepted: September 10, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Many studies reported reduced antioxidant capacity in the vasculature under hypertensive conditions. However, little is known about the effects of antihypertensive treatments on the regulation of vascular antioxidant enzymes. Thus, we hypothesized that antihypertensive treatments prevent the reduction of antioxidant enzyme activity and expression in the small vessels of angiotensin II-induced hypertensive rats (ANG). We observed the small mesenteric arteries and small renal vessels of normotensive rats (NORM), ANG, and ANG treated with a triple antihypertensive therapy of reserpine, hydrochlorothiazide, and hydralazine (ANG + TTx). Systolic blood pressure was increased in ANG, which was attenuated by 2 weeks of triple therapy (127, 191, and 143 mmHg for NORM, ANG, and ANG + TTx, respectively; p < 0.05). Total superoxide dismutase (SOD) activity in the small mesenteric arteries of ANG was lower than that of NORM. The protein expression of SOD1 was lower in ANG than in NORM, whereas SOD2 and SOD3 expression was not different between the groups. Reduced SOD activity and SOD1 expression in ANG was not restored in ANG + TTx. Both SOD activity and SOD isoform expression in the small renal vessels of ANG were not different from those of NORM. Interestingly, SOD activity in the small renal vessels was reduced by TTx. Between groups, there was no difference in catalase activity or expression in both the small mesenteric arteries and small renal vessels. In conclusion, SOD activity in the small mesenteric arteries decreased by angiotensin II administration, but not by hypertension, which is caused by decreased SOD1 expression.
Keywords : Superoxide dismutase, Angiotensin II, Triple antihypertensive therapy, Small vessels


e-submission

This Article


Cited By Articles
  • CrossRef (0)

Services

Archives