Alterations of Antioxidant Status and Mitochondrial Succinate Dehydrogenase Activity in the Liver of Wistar Strain Albino Rats Treated with by Ethanol Extracts of Annona senegalensis Pers (Annonaceae) Stem Bark
Toxicological Research 2019;35:13−24
Published online January 15, 2019;  https://doi.org/10.5487/TR.2019.35.1.013
© 2019 Korean Society of Toxicology.

Rahmat Adetutu Adisa1, Naimat Kolawole1, Lateef A., Sulaimon1, Bathlomew Brai2 and Abraham Ijaola1

1Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Idi-araba, University of Lagos, Lagos, Nigeria, 2Federal University, Oye -Ekiti, Ekiti State, Nigeria
Rahmat Adetutu Adisa, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Idi-araba, University of Lagos, P.M.B. 12003, Lagos, Nigeria, E-mail: radisa22@yahoo.com; radisa@unilag.edu.ng.com
Received: March 19, 2018; Revised: June 29, 2018; Accepted: July 20, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Numerous ethnomedicinal uses have been attributed to different parts of Annona senegalensis (ASE), including its uses as food and food additives. The present study investigated toxicological and antioxidant effects of 28 days administration of ethanol extracts of ASE stem bark to Wistar strain albino rats. Acute toxicity test was done to determine lethal dose in Wistar rats while sub- acute toxicity test was conducted on rats divided into four groups (A - control, B - 50 mg/kg, C - 100 mg/kg, D - 150 mg/kg, respectively and treated for 28 days. Oxidative stress markers in liver and kidney as well as hepatic succinate dehydrogenase activity in the mitochondrial and post mitochondrial fractions (PMF) were evaluated. The LD50 value of ASE was > 2,000 mg/kg. White blood cell counts gradually increased, but red blood cell counts and haematocrits level decreased significantly (p <0.05) by about 50%. Liver enzymes in the serum and mitochondrial succinate dehydrogenase activity increased significantly (p <0.05). Superoxide dismutase and catalase activities also increased in liver mitochondria and PMF while malondialdehyde (MDA) and reduced glutathione levels increased only in the PMF. Furthermore, only MDA levels increased significantly in the kidney after 28 days extract administration. Histopathological examination showed hepatic necrosis and no obvious signs of nephrotoxicity. Anona senegalensis is relatively safe, but prolonged ingestion could induce oxidative stress and impair ATP synthesis through the modulation of the activity of mitochondrial succinate dehydrogenase.
Keywords : Anona senegalensis, Toxicity, Oxidative stress, Mitochondrial membrane succinate dehydrogenase


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