A 90-Day Repeated Oral Dose Toxicity Study of Alismatis Rhizoma Aqueous Extract in Rats
Toxicol. Res. 2019;35:191−200
Published online April 15, 2019;  https://doi.org/10.5487/TR.2019.35.2.191
© 2019 Korean Society of Toxicology.

Mu-Jin Lee1,2, Ho-Kyung Jung1, Ki-Ho Lee1, Ji-Hun Jang1, Mi-Ok Sim1, Tea-Gyeong Seong1, Byung-Kwan Ahn1, Jin-Han Shon1, Seong-Ho Ham1, Hyun-Woo Cho1, Yong-Min Kim3, Sung-Jin Park3, Ji-Young Yoon4, Je-Won Ko2 and Jong-Choon Kim2

1Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine, Jangheung, Korea
2College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, Gwangju, Korea
3Preclinical Research Center, MEDVILL Co., Seoul, Korea
4Jeonju AgroBio-Materials Institute, Jeonju, Korea
Jong-Choon Kim, College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea
E-mail: toxkim@jnu.ac.kr
Hyun-Woo Cho, Division of Tradition Korean Medicine Research, National Development Institute of Korean Medicine, 288 Udeuraendeugil, Anyang-myeon, Jangheung, Jeonnam 59338, Korea
E-mail: johw7@nikom.or.kr
Received: August 22, 2018; Accepted: November 6, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Alismatis rhizoma (AR), the dried rhizome of Alisma orientale (Sam.) Juzep, is a well-known, traditional medicine that is used for the various biological activities including as a diuretic, to lower cholesterol and as an anti-inflammatory agent. The present study was carried out to investigate the potential toxicity of the Alismatis rhizoma aqueous extract (ARAE) following 90-day repeated oral administration to Sprague-Dawley rats. ARAE was administered orally to male and female rats for 90 days at 0 (control), 500, 1,000 and 2,000 mg/kg/day (n = 10 for male and female rats for each dose). Additional recovery groups from the control group and high dose group were observed for a 28-day recovery period. Chromatograms of ARAE detected main compounds with four peaks. Treatment-related effects including an increase in the red blood cells, hemoglobin, hematocrit, albumin, total protein, and urine volume were observed in males of the 2,000 mg/kg/day group (p < 0.05). However, the diuretic effect of ARAE was considered, a major cause of hematological and serum biochemical changes. The oral no-observed-adverse-effect level (NOAEL) of the ARAE was > 2,000 mg/kg/day in both genders, and no target organs were identified.
Keywords : Alismatis rhizome aqueous extract, Traditional medicine, Diuretic effect, Sub-chronic toxicity, NOAEL


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